Siha细胞

细胞名称： 人子宫颈鳞癌细胞  Siha细胞

细胞别称：Siha; SIHA

货号：TCH-C326

规格： 1X10^6

价格：1400元 (干冰运输需另加200元干冰费)

种属来源：人

组织来源：宫颈

特征：宫颈鳞癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%P/S

配套培养基货号：TCH-G326

传代比例：1:3-1:6，每2-3天换液一次

Siha细胞传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

  Siha细胞来源于一位日本的手术切除的肿瘤组织。电镜下可以看到细胞间典型的桥粒和细胞质中大量的张力丝。1975年发现有支原体污染，而后被去除。该细胞整合有HPV16基因组，每个细胞中有1~2个拷贝。

    苏州阿尔法生物除了提供细胞系购买，细胞株购买，肺癌细胞株，ht-29细胞 ，血管内皮细胞株，肝癌细胞株，肿瘤细胞株，胃癌细胞株，hela细胞株，Siha细胞，s180细胞株，hela细胞株，CHO细胞株，间充质干细胞，还提供MEM培养基,DMEM,F12培养基,RPMI培养基等基础培养基，干细胞培养基,培养基添加剂,间充质干细胞培养基 ,完全培养基,低血清培养基,胎牛血清,鸡血清等细胞培养试剂以及实验耗材，实验室仪器等。

参考文献：

"Annunziata C, et al. Distinct profiles of TERT promoter mutations and telomerase expression in head and neck cancer and cervical carcinoma. Int J Cancer 143(5): 1153-1161, 2018. PubMed: 29603728

Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Friedl F, et al. Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proc. Soc. Exp. Biol. Med. 135: 543-545, 1970. PubMed: 5529598

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Olive PL, Banath JP. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res. 57: 5528-5533, 1997. PubMed: 9407963"