人视网膜色素上皮细胞 ARPE19

人视网膜色素上皮细胞 ARPE19 该细胞系系源自于一名19岁车祸罹难的健康男性的视网膜组织，由AmyAotaki-Keen建系于1986年。该细胞系表达视网膜色素细胞特有的分子标记如胞内视黄醛结合蛋白和PRE-65。 

细胞名称：人视网膜色素上皮细胞

细胞简称：ARPE-19

细胞别称：ARPE19; Adult Retinal Pigment Epithelial cell line-19; NTC-200; NTC200

产品货号：TCH-C127

规格：1×10⁶cells  T25细胞培养瓶

种属来源：人

组织来源：视网膜

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DF-12+10%FBS+1%P/S

配套培养基货号：TCH-G127

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

实验室仪器及实验耗材：二氧化碳培养箱，细胞培养皿，细胞培养瓶，细胞培养摇瓶

人视网膜色素上皮细胞 ARPE19 使用说明书

参考文献：

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Janssen JJ, et al. Retinoic acid delays transcription of human retinal pigment neuroepithelium marker genes in ARPE-19 cells. Neuroreport 11: 1571-1579, 2000. PubMed: 10841379

Udono T, et al. Adrenomedullin in cultured human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1962-1970, 2000. PubMed: 10845623

Sparrow JR, et al. The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1981-1989, 2000. PubMed: 10845625"