人宫颈癌细胞-HeLa细胞

人宫颈癌细胞-HeLa细胞是一个来自人体组织经连续培养获得的非整倍体上皮样细胞系，它由GeyGO等在1951年从31岁女性黑人的宫颈癌组织建立。已知该细胞系含有人HPV病毒HPV18序列，需在2级生物安全防护台操作。该细胞角蛋白阳性，p53表达量较低，但表达正常水平的pRB（视网膜母细胞瘤抑制因子）。

细胞名称：人宫颈癌细胞

细胞简称：HeLa 细胞

细胞别称：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri

产品货号：TCH-C193

规格：1×10⁶cells，  T25 培养瓶

种属来源：人

组织来源：宫颈

特征：宫颈癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：MEM+10%FBS+1%P/S

人宫颈癌细胞-HeLa细胞 配套培养基货号： TCH-G193

传代比例：1:2-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

实验室仪器：二氧化碳培养箱

实验耗材：T25细胞培养瓶，T75培养瓶,锥形瓶

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