Hep3B2.1-7-人肝癌细胞

 Hep3B2.1-7 人肝癌细胞

    Hep-3b细胞建系于1979年，源自一位患有肝癌的7岁黑人男童。该细胞产生甲胎蛋白（alpha-fetoprotein)、乙肝表面抗原（BHsAg)、白蛋白、巨球蛋白、α-抗胰蛋白酶（alphal-antitrypsin)、转铁蛋白、补体（C3)、C3活性载体，纤维原等，具有广泛的研究价值。

细胞名称： Hep3B2.1-7 人肝癌细胞

细胞简称：Hep3B2.1-7

产品货号：TCH-C195

种属来源：人

组织来源：肝脏

特征：肝癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S

人肝癌细胞  Hep3B2.1-7培养基货号：TCH-G195

传代比例：1:2-1:3，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983

Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137

Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983