mcf7细胞

细胞名称： 人乳腺癌细胞（STR鉴定正确）

细胞简称： MCF-7细胞

细胞别名： MCF 7; MCF.7; MCF-7；MCF7; Michigan Cancer Foundation-7; ssMCF-7;  ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL；

mcf7细胞货号：TCH-C247

规格：1×10⁶cells/T25 培养瓶

参考价格：1500元  (干冰运输需另加200元干冰费) 

种属来源：人

组织来源：乳腺

特征：乳腺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系： MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+10 µg/mL Human recombinant insulin+1%P/S

mcf7细胞 培养基 货号：TCH-G247

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

mcf7细胞 的生长受到肿瘤坏死因子α（TNF alpha）的抑制。可以通过用抗雌激素治疗来调节IGFBP的分泌。

参考文献：

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

Takahashi K, Suzuki K. Association of insulin-like growth-factor-I-induced DNA synthesis with phosphorylation and nuclear exclusion of p53 in human breast cancer MCF-7 cells. Int. J. Cancer 55: 453-458, 1993. PubMed: 8375929

Brandes LJ, Hermonat MW. Receptor status and subsequent sensitivity of subclones of MCF-7 human breast cancer cells surviving exposure to diethylstilbestrol. Cancer Res. 43: 2831-2835, 1983. PubMed: 6850594

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Pratt SE, Pollak MN. Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993. PubMed: 7693333

Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088