L-929细胞-小鼠成纤维细胞

细胞名称：L-929细胞-小鼠成纤维细胞

细胞别称： NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); 

Clone 929; Earles's cells; Earle's L cells

规格： 1X10^6| T25培养瓶

价格：1500元 (干冰运输需另加200元干冰费)

种属来源：小鼠

组织来源：皮下结缔组织

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养体系：MEM+10%FBS+1%P/S

L-929细胞-小鼠成纤维细胞 配套培养基货号：TCM-G749

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

      1948年三月建立了NCTCclone929(小鼠结缔组织)，细胞系L的克隆。细胞系L是较早建立的连续培养细胞系之一，而clone929是较早的克隆株。从一只100日龄的雄性C3H/An小鼠的正常皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L。第95代的细胞系L使用毛细管法分离单细胞建立了clone929。

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