16年专注生物实验室仪器设备&耗材综合供应
0512-6295610418934597460
0512-6295610418934597460
细胞名称：L-929细胞-小鼠成纤维细胞
细胞别称： NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells
规格： 1X10^6| T25培养瓶
价格：1500元 (干冰运输需另加200元干冰费)
L-929细胞-小鼠成纤维细胞 配套培养基货号：TCM-G749
0512-62956104
L-929细胞-小鼠成纤维细胞
细胞名称：L-929细胞-小鼠成纤维细胞
细胞别称： NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC);
Clone 929; Earles's cells; Earle's L cells
规格： 1X10^6| T25培养瓶
价格：1500元 (干冰运输需另加200元干冰费)
种属来源：小鼠
组织来源：皮下结缔组织
细胞形态：成纤维细胞样
生长特性：贴壁生长
培养体系：MEM+10%FBS+1%P/S
L-929细胞-小鼠成纤维细胞 配套培养基货号：TCM-G749
传代比例：1:3-1:6，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
1948年三月建立了NCTCclone929(小鼠结缔组织)，细胞系L的克隆。细胞系L是较早建立的连续培养细胞系之一，而clone929是较早的克隆株。从一只100日龄的雄性C3H/An小鼠的正常皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L。第95代的细胞系L使用毛细管法分离单细胞建立了clone929。
"Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324
Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517
Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311
ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.
ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006).
U.S. Pharmacopeia General Chapters:<87>Biological Reactivity Tests, in vitro. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820
Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943."
当您来到这个页面时，您已成为我们最尊贵的客户，苏州阿尔法生物实验器材有限公司真诚欢迎您的到来！
0512-62956104
十四年专注于生物实验室仪器供应
全国服务热线
