Jurkat细胞

细胞名称： 人T淋巴细胞白血*细胞（STR鉴定正确）

细胞简称：Jurkat细胞

细胞别称：JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1

货号：TCH-C225

规格： 1×10⁶cells/T25 培养瓶

价格：1500元   (干冰运输需另加200元干冰费)

种属来源：人

组织来源：外周血

特征：急性T淋巴细胞白血*

细胞形态：淋巴母细胞样

生长特性：悬浮生长

培养体系：RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Jurkat细胞 配套培养基货号：TCH-G225

传代比例：1:3-1:6，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

该细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。生长方式为悬浮生长，生长速度较快，使用1640培养液培养。显微镜下观察，正常细胞状态为圆润透亮，似鱼卵。

Jurkat细胞株来源于一个14岁男孩的外周血；经弗波酯和外源凝集素或抗T3单克隆抗体中的一种诱导后可产生大量IL-2(IL-2的产生需两种类型的诱导剂)；表达T细胞受体、CD3。

    苏州阿尔法生物实验器材有限公司除了提供细胞系购买，细胞株购买，pc12细胞株，肺癌细胞株，Jurkat细胞，血管内皮细胞株，肝癌细胞株，肿瘤细胞株，胃癌细胞株，hela细胞株，胰岛细胞株，s180细胞株，hela细胞株，动物细胞，干细胞外，还提供MEM培养基,DMEM,F12培养基,RPMI培养基等基础培养基，干细胞培养基,培养基添加剂,间充质干细胞培养基 ,完全培养基,低血清培养基,胎牛血清,鸡血清等细胞培养试剂。

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