hela 细胞

细胞名称： hela 细胞 （STR鉴定正确）

细胞别称：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri

货号：TCH-C193

规格： 1X10^6

细胞简称：HeLa

种属来源：人

组织来源：宫颈

疾病特征：宫颈癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：MEM+10%FBS+1%P/S

配套培养基货号：TCH-G193

传代比例：1:2-1:6，每2-3天换液一次

hela 细胞传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

        hela 细胞是一个来自人体组织经连续培养获得的非整倍体上皮样细胞系，它由GeyGO等在1951年从31岁女性黑人的宫颈癌组织建立。已知该细胞系含有人乳头状瘤病毒HPV18序列，需在2级生物安全防护台操作。该细胞角蛋白阳性，p53表达量较低，但表达正常水平的pRB（视网膜母细胞瘤抑制因子）。

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