hela 细胞

hela 细胞

细胞名称： hela 细胞 （STR鉴定正确）

细胞别称：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri

货号：TCH-C193

规格： 1X10^6

细胞简称：HeLa

种属来源：人

组织来源：宫颈

疾病特征：宫颈癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：MEM+10%FBS+1%P/S

配套培养基货号：TCH-G193

传代比例：1:2-1:6，每2-3天换液一次

hela 细胞传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

        hela 细胞是一个来自人体组织经连续培养获得的非整倍体上皮样细胞系，它由GeyGO等在1951年从31岁女性黑人的宫颈癌组织建立。已知该细胞系含有人乳头状瘤病毒HPV18序列，需在2级生物安全防护台操作。该细胞角蛋白阳性，p53表达量较低，但表达正常水平的pRB（视网膜母细胞瘤抑制因子）。

参考文献：

"American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

AOAC International Invasiveness by Escherichia coli of mammalian cells, microbiological method. Gaithersburg, MD:AOAC International;AOAC ""Official Methods of Analysis of the AOAC International"" 982.36.

Baldi A, et al. Genomic structure of the human retinoblastoma-related Rb2/p130 gene. Proc. Natl. Acad. Sci. USA 93: 4629-4632, 1996. PubMed: 8643454

Gey GO, et al. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12: 264-265, 1952.

Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844

Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740

Schneider-Gadicke A, Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5: 2285-2292, 1986. PubMed: 3023067

Schwarz E, et al. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314: 111-114, 1985. PubMed: 2983228

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Jones HW Jr., et al. George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin. Obstet. Gynecol. 38: 945-949, 1971. PubMed: 4942173"