hela 细胞
细胞名称: hela 细胞 (STR鉴定正确)
细胞别称:HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri
货号:TCH-C193
规格: 1X10^6
细胞简称:HeLa
种属来源:人
组织来源:宫颈
疾病特征:宫颈癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:MEM+10%FBS+1%P/S
配套培养基货号:TCH-G193
传代比例:1:2-1:6,每2-3天换液一次
hela 细胞传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
hela 细胞是一个来自人体组织经连续培养获得的非整倍体上皮样细胞系,它由GeyGO等在1951年从31岁女性黑人的宫颈癌组织建立。已知该细胞系含有人乳头状瘤病毒HPV18序列,需在2级生物安全防护台操作。该细胞角蛋白阳性,p53表达量较低,但表达正常水平的pRB(视网膜母细胞瘤抑制因子)。
参考文献:
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Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740
Schneider-Gadicke A, Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5: 2285-2292, 1986. PubMed: 3023067
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Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
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