CHO-K1细胞-悬浮培养细胞

    CHO-K1细胞-悬浮培养细胞是指将复苏CHO-K1细胞转入T25 cm2细胞培养瓶，以F12K基础培养基添加10%血清培养，待细胞长满单层后，加入 0.25 % 胰蛋白酶消化，传代至装有完全培养基的T25 cm2细胞培养瓶（Corning）中继续培养，当细胞铺满瓶底时，即得贴壁 CHO-K1 细胞。取贴壁CHO-K1 细胞，换液，加入15 mL含血清的完全基和 15 mL无血清CHO-K1专用培养基，2天后吸出一半培养液，加入等量的无血清CHO-K1，每2天重复一次此操作，直到胎牛血清浓度低于 1 %后，以基础培养基B001 继续培养，即培养基中血清浓度依次以 5 %，2 .5 %，1.25%，0.625 %，0 %降低。细胞在无血清CHO-K1专用培养基中密度达到5 ×106cells/ mL时，即得悬浮 CHO-K1 细胞。培养条件：传代培养条件：37±1℃，120rmp，6.0%CO2

细胞名称：仓鼠卵巢细胞（悬浮）

细胞简称：CHO-K1细胞-悬浮培养细胞

产品货号：TCO-C047

种属来源：仓鼠

组织来源：卵巢

细胞形态：圆形

生长特性： 悬浮生长

培养体系：CD CHO Medium+Anti-Clumping Agent+L-Glutamine Solution

配套培养基货号：TCO-G047

传代比例：传代密度：0.2~0.5×106cells/mL；每2-4天传代一次

传代周期：48-72 h

培养条件：气相：94%空气+6%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

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