bv2小胶质细胞

细胞名称： 小鼠小胶质细胞

细胞别称： BV-2

细胞简称：bv2小胶质细胞

货号：TCM-C718

规格： 1X10^6

价格：1500元 (干冰运输需另加200元干冰费)

种属来源：小鼠

组织来源：脑

细胞形态：多形型

生长特性：半贴壁生长

培养体系：DMEM-H+10%FBS+1%P/S

bv2小胶质细胞 配套培养基货号：TCM-G718

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

bv2小胶质细胞 要表达核v-myc、染色体v-raf癌基因，表面表达envgp70抗原，在形态学、表型及功能上有吞噬细胞的特征。

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参考文献：

"Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

Stansley B., Post J., Hensley K.

A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.

J. Neuroinflamm. 9:115-115(2012)

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14-14(2011)

Crain J.M., Watters J.J.

Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.

Open Drug Discov. J. 2:148-167(2010)

Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.

The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.

ALTEX 26:83-94(2009)"