PC-12细胞

细胞名称： 大鼠肾上腺嗜铬细胞瘤细胞(未分化)

细胞别名：PC-12; PC 12

货号：TCR-C617

规格： 1X10^6

价格：1500 元（推荐T25瓶，复苏发货1-2周）

该细胞系来自可移植的雄性大鼠的肾上腺嗜铬细胞瘤；表达神经生长因子(NGF)受体，NGF可诱导该细胞产生可逆性的神经表型。该细胞可产生儿茶酚胺、多巴胺和去甲肾上腺素，但不合成肾上腺素。

种属来源：大鼠

组织来源：肾上腺

疾病特征：嗜铬细胞瘤

细胞形态：圆形

生长特性：疏松贴壁和悬浮同时存在

培养体系：RPMI-1640+5%FBS+15%马血清+1%P/S

配套培养基货号：TCR-G617

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897