3t3-l1 细胞

细胞名称：3t3-l1 细胞Ppp2r1b基因敲除株

细胞简称：3T3-L1 KO-Ppp2r1b

产品货号：CGKO-M2175

参考价格：15187元

修饰基因：Ppp2r1b Gene (ID: 73699)

修饰类型：敲除

转导方法：慢病毒转导

抗性基因：Hygro

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：小鼠

组织来源：胚胎

细胞形态：成纤维细胞样

生长特性：贴壁生长

3t3-l1 细胞培养体系：DMEM-H+10%CS（新生牛血清）+1%P/S

传代比例：1:3-1:4，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

  3t3-l1 细胞 是从3T3细胞（Swissalbino）中经克隆分离得到的连续传代的亚系。该细胞从快速分裂到汇合和接触性抑制状态经历了前脂肪细胞到脂肪样细胞的转变。该细胞鼠痘病毒阴性；可产生甘油三酯，高浓度血清可增强细胞内脂肪堆积.

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